COL6A1 Recombinant antibody, PBS Only

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  WB analysis using 85704-1-RR (same clone as 85704-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85704-1-RR (COL6A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85704-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 85704-1-RR (same clone as 85704-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 85704-1-RR (COL6A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85704-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85704-1-RR (same clone as 85704-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85704-1-RR (COL6A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85704-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 85704-1-RR (same clone as 85704-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85704-1-RR (Collagen Type VI antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85704-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 85704-1-RR (same clone as 85704-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85704-1-RR (Collagen Type VI antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85704-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 85704-1-RR (same clone as 85704-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85704-1-RR (COL6A1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85704-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 85704-1-RR (same clone as 85704-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85704-1-RR (COL6A1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85704-1-PBS in a different storage buffer formulation.

COL6A1 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 85704-1-PBS

Clone No.242936H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

COL6, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain, Collagen Type VI

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Product Information

85704-1-PBS targets COL6A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: COL6A1 fusion protein Ag11809
• Full Name: collagen, type VI, alpha 1
• Calculated Molecular Weight: 1028 aa, 109 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC052575
• Gene Symbol: COL6A1
• Gene ID (NCBI): 1291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P12109
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.