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  • Western Blot (WB) analysis of various lysates using C1QA Recombinant antibody (85719-4-RR)

    WB analysis using 85719-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85719-4-RR (C1QA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C1QA Recombinant antibody (85719-4-RR)

    IF Staining of HepG2 using 85719-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C1QA antibody (85719-4-RR, Clone: 250093H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

C1QA Recombinant antibody

C1QA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85719-4-RR
Clone No.250093H1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Complement C1q A chain

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inmouse spleen tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85719-4-RR targets C1QA in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen C1QA fusion protein Eg2396 Predict reactive species
Full Name complement component 1, q subcomponent, A chain
Calculated Molecular Weight26kDa
Observed Molecular Weight 26-30 kDa
GenBank Accession NumberNM_015991.4
Gene Symbol C1QA
Gene ID (NCBI) 712
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02745
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.

Protocols

Product Specific Protocols
WB protocol for C1QA antibody 85719-4-RRDownload protocol
IF protocol for C1QA antibody 85719-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols