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Tested Applications
| Positive WB detected in | rat brain tissue, rat cerebellum tissue |
| Positive IHC detected in | rat cerebellum tissue, mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85740-1-RR targets SCN11A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | sodium channel, voltage-gated, type XI, alpha subunit |
| Calculated Molecular Weight | 205 kDa |
| Observed Molecular Weight | 205 kDa |
| GenBank Accession Number | NM_001349253 |
| Gene Symbol | SCN11A |
| Gene ID (NCBI) | 11280 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UI33 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Sodium channel protein type 11 subunit alpha (SCN11A, also known as Nav1.9) is crucial for generating action potentials and regulating neuronal excitability, particularly in nociceptive neurons of the dorsal root ganglia (DRG) and trigeminal ganglia (PMID: 33752606). Nav1.9 channels are involved in the formation of pain-sensing and have been implicated in peripheral inflammatory pain hypersensitivity. Additionally, SCN11A-mediated channels play a role in regulating colonic motility and neurotransmitter release in the enteric nervous system (PMID: 35711274).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SCN11A antibody 85740-1-RR | Download protocol |
| WB protocol for SCN11A antibody 85740-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
