Various lysates were subjected to SDS PAGE followed by western blot with 85770-1-RR (Cleaved Caspase 4/P20 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 85770-1-RR (Cleaved Caspase 4/P20 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Caspase-4 participates in cleavage and activates gasdermin D (GSDMD), leading to cell membrane perforation, cell swelling and rupture, thereby inducing pyroptosis.
Protocols
Product Specific Protocols
WB protocol for Cleaved Caspase 4/P20 antibody 85770-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 85770-1-RR (Cleaved Caspase 4/P20 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Cleaved Caspase 4/P20 Recombinant antibody
Catalog Number
85770-1-RR
Citations
-
Dilutions
WB : 1:1000-1:4000
Applications
WB, ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
