Tested Applications
| Positive WB detected in | Staurosporine treated Jurkat cells, RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85770-1-RR targets Cleaved Caspase 4/P20 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | caspase 4, apoptosis-related cysteine peptidase |
| Calculated Molecular Weight | 377 aa, 43 kDa |
| Observed Molecular Weight | 20 kDa |
| GenBank Accession Number | BC017839 |
| Gene Symbol | Caspase 4 |
| Gene ID (NCBI) | 837 |
| RRID | AB_3744362 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P49662 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Caspase-4 participates in cleavage and activates gasdermin D (GSDMD), leading to cell membrane perforation, cell swelling and rupture, thereby inducing pyroptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cleaved Caspase 4/P20 antibody 85770-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Cleaved Caspase 4/P20 Recombinant monoclonal antibody (243150D6), catalog number 85770-1-RR, has 1 total citation. The citation appears in Cell Reports (2026), a study investigating how the pyroptosis cascade between inflammatory endothelial cells and microglia facilitates blood-brain barrier disruption in bacterial meningitis. The associated research fields include pyroptosis, neuroinflammation, and infectious disease pathogenesis.
| Species | Application | Title |
|---|---|---|
Cell Rep The pyroptosis cascade between inflammatory endothelial cells and microglia facilitates BBB disruption in bacterial meningitis. |
