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  • Western Blot (WB) analysis of various lysates using hCG Beta Recombinant antibody (85800-7-RR)

    WB analysis using 85800-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85800-7-RR (hCG Beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using hCG Beta Recombinant antibody (85800-7-RR)

    IHC staining of human placenta using 85800-7-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85800-7-RR (hCG Beta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using hCG Beta Recombinant antibody (85800-7-RR)

    IHC staining of human placenta using 85800-7-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85800-7-RR (hCG Beta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

hCG Beta Recombinant antibody

hCG Beta Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85800-7-RR
Clone No.250118E6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CGB, CGB,hCG, CG beta, CGB3, CGbeta

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inJAR cells, human placenta tisse
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85800-7-RR targets hCG Beta in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen hCG Beta fusion protein Eg3378 Predict reactive species
Full Name CG beta
Observed Molecular Weight20-25 kDa
Gene Symbol hCG Beta
Gene ID (NCBI) 1082
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP0DN86
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-hCG (beta-human chorionic gonadotropin) is a glycoprotein hormone produced by the placenta during pregnancy. It is a critical marker used in both obstetrics and oncology for various diagnostic and monitoring purposes. hCG is composed of two subunits: an alpha subunit (similar to other glycoprotein hormones like LH, FSH, and TSH) and a unique beta subunit (β-hCG). The beta subunit is the primary focus for diagnostic tests due to its specificity. β-hCG is initially secreted by the syncytiotrophoblast cells surrounding the developing embryo and later by the placenta.

Protocols

Product Specific Protocols
WB protocol for hCG Beta antibody 85800-7-RRDownload protocol
IHC protocol for hCG Beta antibody 85800-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols