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  • Western Blot (WB) analysis of various lysates using PNO1 Recombinant antibody (85834-4-RR)

    WB analysis using 85834-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85834-4-RR (PNO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using PNO1 Recombinant antibody (85834-4-RR)

    IF Staining of A549 using 85834-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using PNO1 antibody (85834-4-RR, Clone: 250134F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

PNO1 Recombinant antibody

PNO1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85834-4-RR
Clone No.250134F6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

KHRBP1, Partner of NOB1, RNA binding protein PNO1, RNA-binding protein PNO1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inK-562 cells, A2780 cells
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85834-4-RR targets PNO1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PNO1 fusion protein Ag14752 Predict reactive species
Full Name partner of NOB1 homolog (S. cerevisiae)
Calculated Molecular Weight 252 aa, 28 kDa
Observed Molecular Weight 28-35 kDa
GenBank Accession NumberBC008304
Gene Symbol PNO1
Gene ID (NCBI) 56902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NRX1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human RNA binding gene partner of NOB1 (PNO1) was firstly isolated from human kidney and encodes 35-kDa protein, named PNO1. PNO1 as a ribosome biogenesis factor that contributes to 40S subunit assembly by binding with its partner NOB1. PNO1 is a highly conserved protein with a K homology (KH) domain at its C-terminal and two putative nuclear localization signals at its N-terminal . PNO1 also had great regulatory function in tumor metabolism. (PMID: 38071381, PMID: 33864661)

Protocols

Product Specific Protocols
WB protocol for PNO1 antibody 85834-4-RRDownload protocol
IF protocol for PNO1 antibody 85834-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols