PNO1 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 85834-4-RR (same clone as 85834-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85834-4-RR (PNO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85834-4-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 85834-4-RR (same clone as 85834-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using PNO1 antibody (85834-4-RR, Clone: 250134F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85834-4-PBS in a different storage buffer formulation.

PNO1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85834-4-PBS

Clone No.250134F6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

KHRBP1, Partner of NOB1, RNA binding protein PNO1, RNA-binding protein PNO1

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Product Information

85834-4-PBS targets PNO1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag14752

  Product name: Recombinant human PNO1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-252 aa of BC008304

  Sequence: MESEMETQSARAEEGFTQVTRKGGRRAKKRQAEQLSAAGEGGDAGRMDTEEARPAKRPVFPPLCGDGLLSGKEETRKIPVPANRYTPLKENWMKIFTPIVEHLGLQIRFNLKSRNVEIRTCKETKDVSALTKAADFVKAFILGFQVEDALALIRLDDLFLESFEITDVKPLKGDHLSRAIGRIAGKGGKTKFTIENVTRTRIVLADVKVHILGSFQNIKMARTAICNLILGNPPSKVYGNIRAVASRSADRF

• Full Name: partner of NOB1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 252 aa, 28 kDa
• Observed Molecular Weight: 28-35 kDa
• GenBank Accession Number: BC008304
• Gene Symbol: PNO1
• Gene ID (NCBI): 56902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NRX1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Human RNA binding gene partner of NOB1 (PNO1) was firstly isolated from human kidney and encodes 35-kDa protein, named PNO1. PNO1 as a ribosome biogenesis factor that contributes to 40S subunit assembly by binding with its partner NOB1. PNO1 is a highly conserved protein with a K homology (KH) domain at its C-terminal and two putative nuclear localization signals at its N-terminal . PNO1 also had great regulatory function in tumor metabolism. (PMID: 38071381, PMID: 33864661)