DNA Recombinant monoclonal antibody

DNA Uni-rAb^® Recombinant Antibody for IHC, FC (Intra), Dot Blot, ELISA

Cat No. 85854-1-RR

Clone No. 250864A1

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

IHC, FC (Intra), Dot Blot, ELISA

Synonyms

dsDNA, ssDNA, circular DNA, double stranded DNA, double-stranded DNA

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

APC CoraLite® Plus 488

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Filter: AllIHCFC (Intra)Dot Blot

IHC staining of human ovary tumor using 85854-1-RR
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 85854-1-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 85854-1-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 85854-1-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HeLa using 85854-1-RR
1x10^6 HeLa cells were intracellularly stained with 0.2 ug N/a Anti-DNA Recombinant Antibody (85854-1-RR, Clone:250864A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Dot Blot experiment using 85854-1-RR
Dot blot analysis was used to confirm the specificity of 85854-1-RR anti-DNA antibody. samples were spotted onto NC and probed with antibody at 5 µg/ml.The amount of sample (μg) spotted is indicated next to each row.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive IHC detected in	human placenta tissue, human ovary tumor tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	HeLa cells
Positive Dot Blot detected in	N/A

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:800-1:3200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.2 ug per 10^6 cells in a 100 µl suspension
DOT BLOT	DOT BLOT : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85854-1-RR targets DNA in IHC, FC (Intra), Dot Blot, ELISA applications and shows reactivity with n/a samples.

Tested Reactivity	n/a
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	DNA Predict reactive species
Full Name	DNA
Gene Symbol
Gene ID (NCBI)
RRID	AB_3744424
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA antibodies are a type of antibody that can specifically bind to DNA and are mainly used to detect and study DNA-related biological processes. This antibody can recognize single-stranded DNA and double-stranded DNA (including linear DNA and circular DNA).

Protocols

Product Specific Protocols
IHC protocol for DNA antibody 85854-1-RR	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The Anti-DNA Antibody (clone 250864A1), catalog number 85854-1-RR, has 1 total citation. It appears in the journal Toxicology, where it was used for IHC, FC, and ELISA to study PFHxS-induced osteoarthritis via innate immune signaling and lipid peroxidation pathways.

Species	Application	Title
	IHC, FC, ELISA	Toxicology PFHxS exposure causes osteoarthritis through the innate immune signaling pathway mediated by lipid peroxidation. Authors - Xicong Chen View Article