DNA Recombinant monoclonal antibody, PBS Only

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• DNA Recombinant monoclonal antibody, PBS Only (85854-1-PBS)

DNA Uni-rAb^® Recombinant Antibody for IHC, FC (Intra), Dot Blot, Indirect ELISA

Cat No. 85854-1-PBS Copied!

Clone No. 250864A1

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Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

IHC, FC (Intra), Dot Blot, Indirect ELISA

Synonyms

dsDNA, ssDNA, circular DNA, double stranded DNA, double-stranded DNA

• 

  IHC staining of human ovary tumor using 85854-1-RR (same clone as 85854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85854-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 85854-1-RR (same clone as 85854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85854-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 85854-1-RR (same clone as 85854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85854-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 85854-1-RR (same clone as 85854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85854-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HeLa using 85854-1-RR (same clone as 85854-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.2 ug N/a Anti-DNA Recombinant Antibody (85854-1-RR, Clone:250864A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 85854-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Dot Blot experiment using 85854-1-RR (same clone as 85854-1-PBS)

  Dot blot analysis was used to confirm the specificity of 85854-1-RR anti-DNA antibody. samples were spotted onto NC and probed with antibody at 5 µg/ml.The amount of sample (μg) spotted is indicated next to each row. This data was developed using the same antibody clone with 85854-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

85854-1-PBS targets DNA in IHC, FC (Intra), Dot Blot, Indirect ELISA applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  DNA

• Full Name: DNA
• RRID: AB_3744424
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DNA antibodies are a type of antibody that can specifically bind to DNA and are mainly used to detect and study DNA-related biological processes. This antibody can recognize single-stranded DNA and double-stranded DNA (including linear DNA and circular DNA).