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  • Western Blot (WB) analysis of rat kidney tissue using SYNJ2BP Recombinant antibody (85870-4-RR)

    WB analysis of rat kidney using 85870-4-RR

    Rat kidney tissue lysates were subjected to SDS PAGE followed by western blot with 85870-4-RR (ARIP2; SYNJ2BP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SYNJ2BP Recombinant antibody (85870-4-RR)

    WB analysis using 85870-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85870-4-RR (ARIP2; SYNJ2BP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SYNJ2BP Recombinant antibody (85870-4-RR)

    IF Staining of HeLa using 85870-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARIP2; SYNJ2BP antibody (85870-4-RR, Clone: 250205D7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SYNJ2BP Recombinant antibody

SYNJ2BP Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85870-4-RR
Clone No.250205D7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Synonyms

ARIP2, OMP25, Synaptojanin-2-binding protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA549 cells, rat kidney tissue, SGC-7901 cells, SH-SY5Y cells, human heart tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85870-4-RR targets SYNJ2BP in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SYNJ2BP fusion protein Ag8130 Predict reactive species
Full Name synaptojanin 2 binding protein
Calculated Molecular Weight 145 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC007704
Gene Symbol SYNJ2BP
Gene ID (NCBI) 55333
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP57105
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Activin, a member of the TGF-beta superfamily, plays important roles in reproductive tissues as a stimulator of follicle-stimulating hormone (FSH) secretion and inhibits the proliferation of breast cancer cells. Activin receptor-interacting protein 2 (ARIP2), also known as synaptojanin-2-binding protein (SYNJ2BP), is located in the outer membrane of mitochondria. SYNJ2BP is widely distributed in various human tissues, and has been recently identified in mouse tissues as a regulatory protein of activin signal transduction by interacting with activin type II receptors. SYNJ2BP may be a putative growth-promoting factor involved in breast tumorigenesis and tumor development. A double band of size 15.9 kDa can be detected in Western blot (PMID: 23284606).

Protocols

Product Specific Protocols
WB protocol for SYNJ2BP antibody 85870-4-RRDownload protocol
IF protocol for SYNJ2BP antibody 85870-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols