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  • Western Blot (WB) analysis of various lysates using TRIM25 Recombinant antibody (85919-1-RR)

    WB analysis using 85919-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85919-1-RR (TRIM25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TRIM25 Recombinant antibody (85919-1-RR)

    IF Staining of A549 using 85919-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using TRIM25 antibody (85919-1-RR, Clone: 250176H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TRIM25 Recombinant antibody

TRIM25 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85919-1-RR
Clone No.250176H6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

EC:2.3.2.27, EC:6.3.2.n3, EFP, Estrogen-responsive finger protein, RING-type E3 ubiquitin transferase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, HepG2 cells
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85919-1-RR targets TRIM25 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRIM25 fusion protein Ag29325 Predict reactive species
Full Name tripartite motif-containing 25
Calculated Molecular Weight 630 aa, 71 kDa
Observed Molecular Weight71 kDa
GenBank Accession NumberBC016924
Gene Symbol TRIM25
Gene ID (NCBI) 7706
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14258
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity(PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection(PMID: 17392790). TRIM25 can be detected in both the cytoplasmic and nuclear (PMID: 33966039).

Protocols

Product Specific Protocols
WB protocol for TRIM25 antibody 85919-1-RRDownload protocol
IF protocol for TRIM25 antibody 85919-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols