TRIM25 Recombinant antibody, PBS Only

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  WB analysis using 85919-1-RR (same clone as 85919-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85919-1-RR (TRIM25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85919-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 85919-1-RR (same clone as 85919-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using TRIM25 antibody (85919-1-RR, Clone: 250176H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85919-1-PBS in a different storage buffer formulation.

TRIM25 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85919-1-PBS

Clone No.250176H6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EC:2.3.2.27, EC:6.3.2.n3, EFP, Estrogen-responsive finger protein, RING-type E3 ubiquitin transferase

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Product Information

85919-1-PBS targets TRIM25 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TRIM25 fusion protein Ag29325
• Full Name: tripartite motif-containing 25
• Calculated Molecular Weight: 630 aa, 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC016924
• Gene Symbol: TRIM25
• Gene ID (NCBI): 7706
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14258
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity(PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection(PMID: 17392790). TRIM25 can be detected in both the cytoplasmic and nuclear (PMID: 33966039).