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  • Western Blot (WB) analysis of PC-12 cells using TH Recombinant antibody (85929-5-RR)

    WB analysis of PC-12 using 85929-5-RR

    PC-12 cells were subjected to SDS PAGE followed by western blot with 85929-5-RR (TH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TH Recombinant antibody (85929-5-RR)

    WB analysis using 85929-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85929-5-RR (TH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TH Recombinant monoclonal antibody (85929-5-RR)

    IHC staining of mouse brain using 85929-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85929-5-RR (TH antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using TH Recombinant monoclonal antibody (85929-5-RR)

    IF Staining of rat brain using 85929-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using TH antibody (85929-5-RR, Clone: 250146D7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TH Recombinant monoclonal antibody (85929-5-RR)

    IF Staining of mouse brain using 85929-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TH antibody (85929-5-RR, Clone: 250146D7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85929-5-RR

    Affinity and Kinetic Characterization of 85929-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85929-5-RR against Human TH were performed. The affinity constant is 47.4 pM.

TH Recombinant monoclonal antibody

TH Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 85929-5-RR
Clone No.250146D7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

tyrosine hydroxylase, DYT14, DYT5b, EC:1.14.16.2, TYH

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inrat brain tissue, PC-12 cells, mouse brain tissue, fetal human brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1250-1:5000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85929-5-RR targets TH in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag23034

Product name: Recombinant human TH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-260 aa of BC104967

Sequence: MPTPDATTPQAKGFRRAVSELDAKQAEAIMSPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLEAVAFEEKEGKAMLNLLFSPRATKPSALSRAVKVFETFEAKIHHLETRPAQRPRAGGPHLEYFVRLEVRRGDLAALLSGVRQVSEDVRSPAGPKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLER

Predict reactive species
Full Name tyrosine hydroxylase
Calculated Molecular Weight 528 aa, 59 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC104967
Gene Symbol TH
Gene ID (NCBI) 7054
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP07101
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons.

Protocols

Product Specific Protocols
IF protocol for TH antibody 85929-5-RRDownload protocol
IHC protocol for TH antibody 85929-5-RRDownload protocol
WB protocol for TH antibody 85929-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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