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TH Recombinant monoclonal antibody, PBS Only (85929-5-PBS)

Filter: AllWBIHCIF-PAffinity

WB analysis of PC-12 using 85929-5-RR (same clone as 85929-5-PBS)
PC-12 cells were subjected to SDS PAGE followed by western blot with 85929-5-RR (TH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85929-5-PBS in a different storage buffer formulation.
WB analysis using 85929-5-RR (same clone as 85929-5-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 85929-5-RR (TH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85929-5-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 85929-5-RR (same clone as 85929-5-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85929-5-RR (TH antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85929-5-PBS in a different storage buffer formulation.
IHC staining of human colon using 85929-5-RR (same clone as 85929-5-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85929-5-RR (TH antibody) at dilution of 1:30000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85929-5-PBS in a different storage buffer formulation.
IHC staining of human colon using 85929-5-RR (same clone as 85929-5-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85929-5-RR (TH antibody) at dilution of 1:30000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85929-5-PBS in a different storage buffer formulation.
IF Staining of rat brain using 85929-5-RR (same clone as 85929-5-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using TH antibody (85929-5-RR, Clone: 250146D7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85929-5-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 85929-5-RR (same clone as 85929-5-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TH antibody (85929-5-RR, Clone: 250146D7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85929-5-PBS in a different storage buffer formulation.
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Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TH antibody (85929-5-RR, Clone: 250146D7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85929-5-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 85929-5-RR
Biolayer interferometry (BLl) kinetic assays of 85929-5-RR against Human TH were performed. The affinity constant is 47.4 pM.

TH Recombinant monoclonal antibody, PBS Only

Name: TH Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 85929-5-PBS

TH Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 85929-5-PBS

Clone No.250146D7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

tyrosine hydroxylase, DYT14, DYT5b, EC:1.14.16.2, TYH

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

85929-5-PBS targets TH in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag23034 Product name: Recombinant human TH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-260 aa of BC104967 Sequence: MPTPDATTPQAKGFRRAVSELDAKQAEAIMSPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLEAVAFEEKEGKAMLNLLFSPRATKPSALSRAVKVFETFEAKIHHLETRPAQRPRAGGPHLEYFVRLEVRRGDLAALLSGVRQVSEDVRSPAGPKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLER Predict reactive species
Full Name	tyrosine hydroxylase
Calculated Molecular Weight	528 aa, 59 kDa
Observed Molecular Weight	60 kDa
GenBank Accession Number	BC104967
Gene Symbol	TH
Gene ID (NCBI)	7054
RRID	AB_3744480
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P07101
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons.