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  • Western Blot (WB) analysis of various lysates using SP7 Recombinant antibody (85934-1-RR)

    WB analysis using 85934-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85934-1-RR (SP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85934-1-RR

    Affinity and Kinetic Characterization of 85934-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85934-1-RR against Human SP7 were performed. The affinity constant is 1.10 nM.

SP7 Recombinant antibody

SP7 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85934-1-RR
Clone No.250286C5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

osterix, OSX, Sp7 transcription factor, Transcription factor Sp7, Zinc finger protein osterix

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inSaos-2 cells, U2OS cells, MG-63 cells, Sp2/0 cells, mouse spinal cord tissue, mouse testis tissue, rat brain tiisue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85934-1-RR targets SP7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SP7 fusion protein Ag29889 Predict reactive species
Full Name Sp7 transcription factor
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberNM_152860
Gene Symbol SP7
Gene ID (NCBI) 121340
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TDD2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sp7 is identified in several tissues, including bone, tooth, brain, and the reproductive tract (PMID: 11792318, 33135214, 36881265). In bone, Sp7 is expressed at multiple stages throughout the mesenchymal bone lineage and plays different roles in different osteocytes (PMID: 36881265). SP7 regulates osteoblast differentiation and bone formation through osteoblast target genes by recognizing AT-rich region. In osteocytes, SP7 regulates osteocyte maturation and intracortical remodeling through osteocyte target genes by recognizing TGA(G/T) TCA motif. SP7 also regulates chondrocyte differentiation and endochondral ossification (36881265).

Protocols

Product Specific Protocols
WB protocol for SP7 antibody 85934-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols