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  • Western Blot (WB) analysis of various lysates using NUMB Recombinant antibody (85939-4-RR)

    WB analysis using 85939-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85939-4-RR (NUMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NUMB Recombinant antibody (85939-4-RR)

    WB analysis using 85939-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85939-4-RR (NUMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using NUMB Recombinant antibody (85939-4-RR)

    IP experiment of HeLa using 85939-4-RR

    IP result of anti-NUMB (IP:85939-4-RR, 4ug; Detection:85939-4-RR 1:3000) with HeLa cells lysate 1600 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NUMB Recombinant antibody (85939-4-RR)

    IHC staining of human breast cancer using 85939-4-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85939-4-RR (NUMB antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NUMB Recombinant antibody (85939-4-RR)

    IHC staining of human breast cancer using 85939-4-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85939-4-RR (NUMB antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85939-4-RR

    Affinity and Kinetic Characterization of 85939-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85939-4-RR against Human NUMB were performed. The affinity constant is 1.15 nM.

NUMB Recombinant antibody

NUMB Uni-rAbTM Recombinant Antibody for WB, IHC, IP, ELISA
Cat No. 85939-4-RR
Clone No.250417F6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

C14orf41, h Numb, h-Numb

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA431 cells, A-204 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue, SKOV-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85939-4-RR targets NUMB in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NUMB fusion protein Ag31553 Predict reactive species
Full Name numb homolog (Drosophila)
Calculated Molecular Weight71KD
Observed Molecular Weight66-71 kDa
GenBank Accession NumberNM_001005743
Gene Symbol NUMB
Gene ID (NCBI) 8650
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49757
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene.

Protocols

Product Specific Protocols
WB protocol for NUMB antibody 85939-4-RRDownload protocol
IHC protocol for NUMB antibody 85939-4-RRDownload protocol
IP protocol for NUMB antibody 85939-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols