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  • Western Blot (WB) analysis of LNCaP cells using CD58 Recombinant monoclonal antibody (85950-1-RR)

    WB analysis of LNCaP using 85950-1-RR

    WB result of CD58 antibody (85950-1-RR; 1:1000; room temperature for 1.5 hours) with wild-type and CD58 knockout LNCaP cells.

  • Western Blot (WB) analysis of various lysates using CD58 Recombinant antibody (85950-1-RR)

    WB analysis using 85950-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85950-1-RR (CD58 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CD58 Recombinant antibody (85950-1-RR)

    WB analysis using 85950-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85950-1-RR (CD58 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD58 Recombinant monoclonal antibody (85950-1-RR)

    IHC staining of human tonsillitis using 85950-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85950-1-RR (CD58 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD58 Recombinant monoclonal antibody (85950-1-RR)

    IHC staining of human tonsillitis using 85950-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85950-1-RR (CD58 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using CD58 Recombinant monoclonal antibody (85950-1-RR)

    IF Staining of K-562 using 85950-1-RR

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD58 antibody (85950-1-RR, Clone: 250250H5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

CD58 Recombinant monoclonal antibody

CD58 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85950-1-RR
Clone No.250250H5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Ag3, CD58 molecule, LFA 3, LFA3, Lymphocyte function-associated antigen 3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inRaji cells, LNCaP cells, Ramos cells, Jurkat cells, K-562 cells, Karpas-299 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85950-1-RR targets CD58 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg4236

Product name: Recombinant Human CD58 protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 29-215 aa of BC005930

Sequence: FSQQIYGVVYGNVTFHVPSNVPLKEVLWKKQKDKVAELENSEFRAFSSFKNRVYLDTVSGSLTIYNLTSSDEDEYEMESPNITDTMKFFLYVLESLPSPTLTCALTNGSIEVQCMIPEHYNSHRGLIMYSWDCPMEQCKRNSTSIYFKMENDLPQKIQCTLSNPLFNTTSSIILTTCIPSSGHSRHR

Predict reactive species
Full Name CD58 molecule
Calculated Molecular Weight 28 kDa
Observed Molecular Weight55-70 kDa
GenBank Accession NumberBC005930
Gene Symbol CD58
Gene ID (NCBI) 965
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP19256
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a heavily glycosylated protein of 55-70 kDa that is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells.

Protocols

Product Specific Protocols
IF protocol for CD58 antibody 85950-1-RRDownload protocol
IHC protocol for CD58 antibody 85950-1-RRDownload protocol
WB protocol for CD58 antibody 85950-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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