CD58 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 85950-1-RR (same clone as 85950-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85950-1-RR (CD58 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85950-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85950-1-RR (same clone as 85950-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85950-1-RR (CD58 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85950-1-PBS in a different storage buffer formulation.

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  Sample test of MP02208-1

  The mean CD58 concentration was determined to be 126.7 ng/mL in human serum.

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  Cytometric bead array standard curve of MP02208-1

  Cytometric bead array standard curve of MP02208-1, CD58 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85950-1-PBS. Detection antibody: 85950-2-PBS. Standard: Eg4236. Range: 0.313-40 ng/mL.

CD58 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA, Sample test

Cat No. 85950-1-PBS

Clone No.250250H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA, Sample test

Synonyms

Ag3, CD58 molecule, LFA 3, LFA3, Lymphocyte function-associated antigen 3

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Product Information

85950-1-PBS targets CD58 as part of a matched antibody pair:

MP02208-1: 85950-1-PBS capture and 85950-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD58 fusion protein Eg4236
• Full Name: CD58 molecule
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 55-70 kDa
• GenBank Accession Number: BC005930
• Gene Symbol: CD58
• Gene ID (NCBI): 965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19256
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a heavily glycosylated protein of 55-70 kDa that is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells.