Sandwich ELISA standard curve of MP02208-2, Human CD58 Recombinant Matched Antibody Pair - PBS only. 85950-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg4236. 85950-2-PBS was HRP conjugated as the detection antibody. Range: 46.9-3000 pg/mL

Serum of sixteen individual healthy human donors was measured. The human CD58 concentration of detected samples was determined to be 25,010.4 pg/mL with a range of 14,622.8 - 36,503.5 pg/mL.

The mean CD58 concentration was determined to be 126.7 ng/mL in human serum.

Urine of eight individual healthy human donors was measured. The human CD58 concentration of detected samples was determined to be 4,388.9 pg/mL with a range of 1,243.7 - 13,113.2 pg/mL.

Raji human Burkitt’s lymphoma cells (5 x 10^6 cells/mL) were cultured in RPMI supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human CD58, and measured 666.0 pg/mL.

The mean CD58 concentration was determined to be 13.50 ng/mL in Jurkat cell extract based on a 2.2 mg/mL extract load and 14.40 ng/mL in human placenta tissue extract based on a 1.7 mg/mL extract load.

Cytometric bead array standard curve of MP02208-1, CD58 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85950-1-PBS. Detection antibody: 85950-2-PBS. Standard: Eg4236. Range: 0.313-40 ng/mL.