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  • Western Blot (WB) analysis of various lysates using GSTA4 Recombinant antibody (85954-2-RR)

    WB analysis using 85954-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85954-2-RR (GSTA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using GSTA4 Recombinant antibody (85954-2-RR)

    IF Staining of HEK-293 using 85954-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using GSTA4 antibody (85954-2-RR, Clone: 250352E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85954-2-RR

    Affinity and Kinetic Characterization of 85954-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85954-2-RR against Human GSTA4 were performed. The affinity constant is 0.489 nM.

GSTA4 Recombinant antibody

GSTA4 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85954-2-RR
Clone No.250352E2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EC:2.5.1.18, Glutathione S transferase A4, Glutathione S transferase A4 4, Glutathione S-transferase A4, Glutathione S-transferase A4-4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, HuH-7 cells, HEK-293T cells, fetal human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85954-2-RR targets GSTA4 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GSTA4 fusion protein Ag11028 Predict reactive species
Full Name glutathione S-transferase alpha 4
Calculated Molecular Weight 222 aa, 27 kDa
Observed Molecular Weight25 kDa
GenBank Accession NumberBC015523
Gene Symbol GSTA4
Gene ID (NCBI) 2941
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15217
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSTA4 (Glutathione S-Transferase Alpha 4) is an enzyme that plays a key role in detoxification and antioxidant defense. Its structure includes a GSH-binding site and a hydrophobic substrate-binding domain, functioning as a dimer. Dysregulation of GSTA4 is linked to diseases like cancer, neurodegeneration, and diabetes.

Protocols

Product Specific Protocols
WB protocol for GSTA4 antibody 85954-2-RRDownload protocol
IF protocol for GSTA4 antibody 85954-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols