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  • Western Blot (WB) analysis of various lysates using PDE3B Recombinant antibody (85996-1-RR)

    WB analysis using 85996-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85996-1-RR (PDE3B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse adipose tissue using PDE3B Recombinant antibody (85996-1-RR)

    WB analysis of mouse adipose using 85996-1-RR

    Mouse adipose tissue were subjected to SDS PAGE followed by western blot with 85996-1-RR (PDE3B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse adipose tissue using PDE3B Recombinant antibody (85996-1-RR)

    IHC staining of mouse adipose using 85996-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse adipose tissue slide using 85996-1-RR (PDE3B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse adipose tissue using PDE3B Recombinant antibody (85996-1-RR)

    IHC staining of mouse adipose using 85996-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse adipose tissue slide using 85996-1-RR (PDE3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85996-1-RR

    Affinity and Kinetic Characterization of 85996-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85996-1-RR against Human PDE3B were performed. The affinity constant is below 1 pM.

PDE3B Recombinant antibody

PDE3B Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85996-1-RR
Clone No.250460H7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

CGIP1, CGI-PDE B, cGIPDE1, cGMP-inhibited 3',5'-cyclic phosphodiesterase 3B, Cyclic GMP-inhibited phosphodiesterase B

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inHeLa cells, mouse adipose tissue, Jurkat cells
Positive IHC detected inmouse adipose tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85996-1-RR targets PDE3B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PDE3B fusion protein Ag19190 Predict reactive species
Full Name phosphodiesterase 3B, cGMP-inhibited
Calculated Molecular Weight 1112 aa, 124 kDa
Observed Molecular Weight 124 kDa
GenBank Accession NumberBC136565
Gene Symbol PDE3B
Gene ID (NCBI) 5140
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13370
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDE3B is a cyclic nucleotide phosphodiesterase with dual specificity for cAMP and cGMP. It plays crucial roles in regulating physiological processes such as angiogenesis and cardiac contractility. PDE3B can be activated by insulin and is involved in the antilipolytic actions of insulin. Additionally, PDE3B forms macromolecular complexes that are important for its activation and interaction with other proteins (PMID: 22001403, PMID: 26031333).

Protocols

Product Specific Protocols
WB protocol for PDE3B antibody 85996-1-RRDownload protocol
IHC protocol for PDE3B antibody 85996-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols