PDE3B Recombinant antibody, PBS Only

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  WB analysis using 85996-1-RR (same clone as 85996-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85996-1-RR (PDE3B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85996-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse adipose using 85996-1-RR (same clone as 85996-1-PBS)

  Mouse adipose tissue were subjected to SDS PAGE followed by western blot with 85996-1-RR (PDE3B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85996-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse adipose using 85996-1-RR (same clone as 85996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse adipose tissue slide using 85996-1-RR (PDE3B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85996-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse adipose using 85996-1-RR (same clone as 85996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse adipose tissue slide using 85996-1-RR (PDE3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85996-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85996-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85996-1-RR against Human PDE3B were performed. The affinity constant is below 1 pM.

PDE3B Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 85996-1-PBS

Clone No.250460H7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

CGIP1, CGI-PDE B, cGIPDE1, cGMP-inhibited 3',5'-cyclic phosphodiesterase 3B, Cyclic GMP-inhibited phosphodiesterase B

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Product Information

85996-1-PBS targets PDE3B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PDE3B fusion protein Ag19190
• Full Name: phosphodiesterase 3B, cGMP-inhibited
• Calculated Molecular Weight: 1112 aa, 124 kDa
• Observed Molecular Weight: 124 kDa
• GenBank Accession Number: BC136565
• Gene Symbol: PDE3B
• Gene ID (NCBI): 5140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13370
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PDE3B is a cyclic nucleotide phosphodiesterase with dual specificity for cAMP and cGMP. It plays crucial roles in regulating physiological processes such as angiogenesis and cardiac contractility. PDE3B can be activated by insulin and is involved in the antilipolytic actions of insulin. Additionally, PDE3B forms macromolecular complexes that are important for its activation and interaction with other proteins (PMID: 22001403, PMID: 26031333).