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  • Western Blot (WB) analysis of various lysates using SERCA2/ATP2A2 Recombinant antibody (86050-1-RR)

    WB analysis using 86050-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86050-1-RR (SERCA2,ATP2A2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SERCA2/ATP2A2 Recombinant antibody (86050-1-RR)

    IHC staining of mouse skeletal muscle using 86050-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 86050-1-RR (SERCA2/ATP2A2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using SERCA2/ATP2A2 Recombinant antibody (86050-1-RR)

    IF Staining of C2C12 using 86050-1-RR

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using SERCA2/ATP2A2 antibody (86050-1-RR, Clone: 250667D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86050-1-RR

    Affinity and Kinetic Characterization of 86050-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86050-1-RR against Human SERCA2/ATP2A2 were performed. The affinity constant is below 1 pM.

SERCA2/ATP2A2 Recombinant antibody

SERCA2/ATP2A2 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 86050-1-RR
Clone No.250667D7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ATP2A2, SERCA2, SERCA2b, ATP2A 2, ATP2B

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected in37°C incubated mouse heart tissue, 37℃ incubated mouse heart, rat heart, 37℃ incubated rat heart
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86050-1-RR targets SERCA2/ATP2A2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SERCA2/ATP2A2 fusion protein Ag26177 Predict reactive species
Full Name ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Calculated Molecular Weight 1042 aa, 115 kDa
Observed Molecular Weight110-115 kDa
GenBank Accession NumberBC035588
Gene Symbol SERCA2
Gene ID (NCBI) 488
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP16615
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.

Protocols

Product Specific Protocols
WB protocol for SERCA2/ATP2A2 antibody 86050-1-RRDownload protocol
IHC protocol for SERCA2/ATP2A2 antibody 86050-1-RRDownload protocol
IF protocol for SERCA2/ATP2A2 antibody 86050-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols