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  • Western Blot (WB) analysis of various lysates using ENDOG Recombinant antibody (86122-1-RR)

    WB analysis using 86122-1-RR (same clone as 86122-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86122-1-RR (ENDOG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86122-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ENDOG Recombinant antibody (86122-1-RR)

    WB analysis using 86122-1-RR (same clone as 86122-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86122-1-RR (ENDOG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86122-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02519-1

    Sandwich ELISA standard curve of MP02519-1

    Sandwich ELISA standard curve of MP02519-1, Human ENDOG Recombinant Matched Antibody Pair - PBS only. 86122-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag17739. 86122-2-PBS was HRP conjugated as the detection antibody. Range: 1.56-50 ng/mL.

  • Sample test of MP02519-1

    Sample test of MP02519-1

    The mean ENDOG concentration was determined to be 1,103.00 ng/mL in Ramos cell extract based on a 1.40 mg/mL extract load, 1,271.00 ng/mL in HepG2 cell extract based on a 1.3 mg/mL extract load and 967.00 ng/mL in HL-60 cell extract based on a 3.7 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ENDOG Recombinant antibody (86122-1-RR)

    IHC staining of mouse kidney using 86122-1-RR (same clone as 86122-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86122-1-RR (ENDOG antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86122-1-PBS in a different storage buffer formulation.

ENDOG Recombinant antibody, PBS Only (Capture)

ENDOG Uni-rAbTM Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 86122-1-PBS
Clone No.250804D2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

EC:3.1.30.-, Endo G, Endonuclease G

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Product Information

86122-1-PBS targets ENDOG as part of a matched antibody pair:

MP02519-1: 86122-1-PBS capture and 86122-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ENDOG fusion protein Ag17739 Predict reactive species
Full Name endonuclease G
Calculated Molecular Weight 297 aa, 33 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC016351
Gene Symbol ENDOG
Gene ID (NCBI) 2021
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ14249
Storage Buffer PBS only, pH 7.3.
Storage ConditionsStore at -80°C.

Background Information

Endonuclease G, also named as EndoG, is a mitochondrial protein. It's a nuclease which was first characterized in bovine heart mitochondrial extracts. It's involved in many cellular process, including apoptosis, paternal mitochondrial elimination and autophage (PMID:33473107). It is a nuclear encoded, sugar-non-specific (PMID:15066427) and well-conserved nuclease (PMID:17244531). It can be released from the mitochondria and translocated to the nucleus where it induces fragmentation of DNA, leading to apoptosis (PMID:11452314). EndoG is a 297-amino-acid long protein with a molecular weight of 30-35 kDa. There is a homodimer form with MW about 60-70 kDa.