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  • Western Blot (WB) analysis of various lysates using TP63 Recombinant antibody (86154-1-RR)

    WB analysis using 86154-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86154-1-RR (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TP63 Recombinant antibody (86154-1-RR)

    IHC staining of human lung cancer using 86154-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86154-1-RR (TP63 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 86154-1-RR

    Affinity and Kinetic Characterization of 86154-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86154-1-RR against Human TP63 were performed. The affinity constant is below 1 pM.

TP63 Recombinant antibody

TP63 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 86154-1-RR
Clone No.250042G4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

p63, CUSP, TP 63, Transformation-related protein 63, Tumor protein 63

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA431 cells, HaCaT cells, mouse skin tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86154-1-RR targets TP63 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TP63 fusion protein Ag2791 Predict reactive species
Full Name tumor protein p63
Calculated Molecular Weight 680 aa, 77 kDa
Observed Molecular Weight63-68 kDa
GenBank Accession NumberBC039815
Gene Symbol TP63
Gene ID (NCBI) 8626
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H3D4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L).

Protocols

Product Specific Protocols
WB protocol for TP63 antibody 86154-1-RRDownload protocol
IHC protocol for TP63 antibody 86154-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols