TP63 Recombinant antibody, PBS Only

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  WB analysis using 86154-1-RR (same clone as 86154-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86154-1-RR (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86154-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 86154-1-RR (same clone as 86154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86154-1-RR (TP63 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86154-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86154-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86154-1-RR against Human TP63 were performed. The affinity constant is below 1 pM.

TP63 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 86154-1-PBS

Clone No.250042G4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

p63, CUSP, TP 63, Transformation-related protein 63, Tumor protein 63

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Product Information

86154-1-PBS targets TP63 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TP63 fusion protein Ag2791
• Full Name: tumor protein p63
• Calculated Molecular Weight: 680 aa, 77 kDa
• Observed Molecular Weight: 63-68 kDa
• GenBank Accession Number: BC039815
• Gene Symbol: TP63
• Gene ID (NCBI): 8626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H3D4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L).