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  • Western Blot (WB) analysis of bEnd.3 cells using CD146/MCAM Recombinant antibody (86159-3-RR)

    WB analysis of bEnd.3 using 86159-3-RR

    bEnd.3 cells were subjected to SDS PAGE followed by western blot with 86159-3-RR (Mcam antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using CD146/MCAM Recombinant antibody (86159-3-RR)

    WB analysis of mouse liver using 86159-3-RR

    mouse liver tissue was subjected to SDS PAGE followed by western blot with 86159-3-RR (Mcam antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of bEnd.3 cells using CD146/MCAM Recombinant antibody (86159-3-RR)

    IF Staining of bEnd.3 using 86159-3-RR

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD146/MCAM antibody (86159-3-RR, Clone: 250806F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CD146/MCAM Recombinant antibody

CD146/MCAM Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86159-3-RR
Clone No.250806F12

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Synonyms

CD146, Cell surface glycoprotein MUC18, Gicerin, Mcam, Melanoma cell adhesion molecule

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inbEnd.3 cells, mouse liver tissue
Positive IF/ICC detected inbEnd.3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86159-3-RR targets CD146/MCAM in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CD146/MCAM fusion protein Eg2667 Predict reactive species
Full Name melanoma cell adhesion molecule
Calculated Molecular Weight72 kDa
Observed Molecular Weight120-140 kDa
GenBank Accession NumberNM_023061.2
Gene Symbol Mcam
Gene ID (NCBI) 84004
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8R2Y2-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

Protocols

Product Specific Protocols
WB protocol for CD146/MCAM antibody 86159-3-RRDownload protocol
IF protocol for CD146/MCAM antibody 86159-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols