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  • Western Blot (WB) analysis of various lysates using AGL Recombinant antibody (86187-1-RR)

    WB analysis using 86187-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86187-1-RR (AGL antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using AGL Recombinant antibody (86187-1-RR)

    WB analysis using 86187-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86187-1-RR (AGL antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86187-1-RR

    Affinity and Kinetic Characterization of 86187-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86187-1-RR against Human AGL were performed. The affinity constant is below 1 pM.

AGL Recombinant monoclonal antibody

AGL Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86187-1-RR
Clone No.250767A6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

4-alpha-glucanotransferase, Amylo-1,6-glucosidase, Amylo-alpha-1,6-glucosidase, Dextrin 6-alpha-D-glucosidase, Glycogen debrancher

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHEK-293 cells, mouse skeletal muscle tissue, Jurkat cells, K-562 cells, rat skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86187-1-RR targets AGL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9962

Product name: Recombinant human AGL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1182-1533 aa of BC078663

Sequence: MYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Predict reactive species
Full Name amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
Calculated Molecular Weight 1532 aa, 175 kDa
Observed Molecular Weight 165 kDa
GenBank Accession NumberBC078663
Gene Symbol AGL
Gene ID (NCBI) 178
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP35573
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Amylo-alpha-1-6-glucosidase-4-alpha-glucanotransferase (AGL) (also known as glycogen debranching enzyme) is a critical bifunctional enzyme involved in glycogen metabolism.

Protocols

Product Specific Protocols
WB protocol for AGL antibody 86187-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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