AGL Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86187-1-RR (same clone as 86187-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86187-1-RR (AGL antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86187-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86187-1-RR (same clone as 86187-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86187-1-RR (AGL antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86187-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86187-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86187-1-RR against Human AGL were performed. The affinity constant is below 1 pM.

AGL Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86187-1-PBS

Clone No.250767A6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

4-alpha-glucanotransferase, Amylo-1,6-glucosidase, Amylo-alpha-1,6-glucosidase, Dextrin 6-alpha-D-glucosidase, Glycogen debrancher

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Product Information

86187-1-PBS targets AGL in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9962

  Product name: Recombinant human AGL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1182-1533 aa of BC078663

  Sequence: MYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

• Full Name: amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
• Calculated Molecular Weight: 1532 aa, 175 kDa
• Observed Molecular Weight: 165 kDa
• GenBank Accession Number: BC078663
• Gene Symbol: AGL
• Gene ID (NCBI): 178
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35573
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Amylo-alpha-1-6-glucosidase-4-alpha-glucanotransferase (AGL) (also known as glycogen debranching enzyme) is a critical bifunctional enzyme involved in glycogen metabolism.