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  • Western Blot (WB) analysis of various lysates using CD25/IL-2R alpha Recombinant antibody (86213-1-RR)

    WB analysis using 86213-1-RR

    Recombinant protein were subjected to SDS PAGE followed by western blot with 86213-1-RR (CD25/IL-2R alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CD25/IL-2R alpha Recombinant antibody (86213-1-RR)

    WB analysis using 86213-1-RR

    CTLL-2 cells were subjected to SDS PAGE followed by western blot with 86213-1-RR (CD25/IL-2R alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

CD25/IL-2R alpha Recombinant antibody

CD25/IL-2R alpha Uni-rAbTM Recombinant Antibody for WB, ELISA

Cat No. 86213-1-RR
Clone No.250670H3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

CD25, IL-2 receptor subunit alpha, IL-2R subunit alpha, Il2ra, IL-2-RA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inCTLL-2 cells, Recombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86213-1-RR targets CD25/IL-2R alpha in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CD25/IL-2R alpha fusion protein Eg1453 Predict reactive species
Full Name interleukin 2 receptor, alpha
Calculated Molecular Weight31kDa
Observed Molecular Weight55-60 kDa
GenBank Accession NumberNM_013163.1
Gene Symbol Il2ra
Gene ID (NCBI) 25704
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26897
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+ Foxp3+ Treg cells (PMID: 22585674).

Protocols

Product Specific Protocols
WB protocol for CD25/IL-2R alpha antibody 86213-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols