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NEK1 Recombinant monoclonal antibody, PBS Only (Capture) (86224-2-PBS)

Filter: AllWBSandwich ELISA sample testSandwich ELISAIF/ICCAffinity

WB analysis using 86224-2-RR (same clone as 86224-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 86224-2-RR (NEK1 antibody) at dilution of 1:21000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86224-2-PBS in a different storage buffer formulation.
WB analysis using 86224-2-RR (same clone as 86224-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 86224-2-RR (NEK1 antibody) at dilution of 1:21000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86224-2-PBS in a different storage buffer formulation.
Sandwich ELISA sample test of MP02331-1
The mean NEK1 concentration was determined to be 0.85 ng/mL in HeLa cell extract based on a 1.30 mg/mL extract load and 0.43 ng/mL in Daudi cell extract based on a 1.8 mg/mL extract load.
Sandwich ELISA standard curve of MP02331-1
Sandwich ELISA standard curve of MP02331-1, Human NEK1 Recombinant Matched Antibody Pair - PBS only. 86224-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag25970. 86224-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.
IF Staining of hTERT-RPE1 using 86224-2-RR (same clone as 86224-2-PBS)
Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using NEK1 antibody (86224-2-RR, Clone: 250128H7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86224-2-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 86224-2-RR
Biolayer interferometry (BLl) kinetic assays of 86224-2-RR against Human NEK1 were performed. The affinity constant is 0.711 nM.

NEK1 Recombinant monoclonal antibody, PBS Only (Capture)

Name: NEK1 Recombinant monoclonal antibody, PBS Only (Capture)
Brand: Proteintech
SKU: 86224-2-PBS

NEK1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Cat No. 86224-2-PBS

Clone No.250128H7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Synonyms

EC:2.7.11.1, KIAA1901, Never in mitosis A-related kinase 1, NimA related protein kinase 1, NimA-related protein kinase 1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

86224-2-PBS targets NEK1 as part of a matched antibody pair:

MP02331-1: 86224-2-PBS capture and 86224-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag25970 Product name: Recombinant human NEK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 557-740 aa of BC114491 Sequence: KRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTD Predict reactive species
Full Name	NIMA (never in mitosis gene a)-related kinase 1
Calculated Molecular Weight	1258 aa, 143 kDa
Observed Molecular Weight	170-180 kDa
GenBank Accession Number	BC114491
Gene Symbol	NEK1
Gene ID (NCBI)	4750
RRID	AB_3744726
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q96PY6
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

NEK1 (NIMA-Related Kinase 1) is a serine/threonine-protein kinase that plays a multifaceted and critical role in cellular homeostasis. It is widely expressed and involved in key biological processes, most notably the DNA Damage Response (DDR) and cilium assembly and function. NEK1 kinase activity is regulated in response to genotoxic stress, where it facilitates the repair of double-strand breaks alongside proteins like ATM and ATR. Simultaneously, it localizes to the basal body of the primary cilium, governing ciliary structure and trafficking. The paramount importance of NEK1 is highlighted by its strong genetic association with Amyotrophic Lateral Sclerosis (ALS); loss-of-function mutations in NEK1 are recognized as one of the most common genetic causes of familial and sporadic ALS. Furthermore, biallelic mutations in NEK1 are responsible for the severe ciliopathy known as short-rib thoracic dysplasia, underscoring its vital role in skeletal development. Thus, NEK1 represents a crucial molecule at the crossroads of genome integrity, ciliary function, and human neurodegenerative and developmental diseases.