Tested Applications
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue, rat brain tissue, fetal human brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86244-1-RR targets Draxin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg4203 Product name: Recombinant Human Draxin protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 26-349 aa of NM_198545.3 Sequence: GALAPGTPARNLPENHIDLPGPALWTPQASHHRRRGPGKKEWGPGLPSQAQDGAVVTATRQASRLPEAEGLLPEQSPAGLLQDKDLLLGLALPYPEKENRPPGWERTRKRSREHKRRRDRLRLHQGRALVRGPSSLMKKAELSEAQVLDAAMEESSTSLAPTMFFLTTFEAAPATEESLILPVTSLRPQQAQPRSDGEVMPTLDMALFDWTDYEDLKPDGWPSAKKKEKHRGKLSSDGNETSPAEGEPCDHHQDCLPGTCCDLREHLCTPHNRGLNNKCFDDCMCVEGLRCYAKFHRNRRVTRRKGRCVEPETANGDQGSFINV Predict reactive species |
| Full Name | chromosome 1 open reading frame 187 |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | NM_198545.3 |
| Gene Symbol | C1orf187 |
| Gene ID (NCBI) | 374946 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8NBI3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Draxin(Dorsal repulsive axon guidance protein) is a repulsive axon guidance cue. It is widely expressed in the developing hippocampus, glioma, and all histological types of the lung cancer cell. Draxin was shown to bind a 22-amino-acid peptide of the EGF domain of Netrin-1, and through Draxin/Netrin-1 interaction, Draxin may act as a secreted Netrin-1 antagonist. C1orf187 can inhibit or repel neurite outgrowth from dorsal spinal cord. It inhibits the stabilization of cytosolic beta-catenin (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist of Wnt signaling pathway (PMID: 29622850).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Draxin antibody 86244-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
