Draxin Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86244-1-RR (same clone as 86244-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86244-1-RR (Draxin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86244-1-PBS in a different storage buffer formulation.

Draxin Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86244-1-PBS

Clone No.250677C12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

C1orf187, AGPA3119, Dorsal inhibitory axon guidance protein, Neucrin, PSEC0258

---

Product Information

86244-1-PBS targets Draxin in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg4203

  Product name: Recombinant Human Draxin protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 26-349 aa of NM_198545.3

  Sequence: GALAPGTPARNLPENHIDLPGPALWTPQASHHRRRGPGKKEWGPGLPSQAQDGAVVTATRQASRLPEAEGLLPEQSPAGLLQDKDLLLGLALPYPEKENRPPGWERTRKRSREHKRRRDRLRLHQGRALVRGPSSLMKKAELSEAQVLDAAMEESSTSLAPTMFFLTTFEAAPATEESLILPVTSLRPQQAQPRSDGEVMPTLDMALFDWTDYEDLKPDGWPSAKKKEKHRGKLSSDGNETSPAEGEPCDHHQDCLPGTCCDLREHLCTPHNRGLNNKCFDDCMCVEGLRCYAKFHRNRRVTRRKGRCVEPETANGDQGSFINV

• Full Name: chromosome 1 open reading frame 187
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_198545.3
• Gene Symbol: C1orf187
• Gene ID (NCBI): 374946
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NBI3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Draxin(Dorsal repulsive axon guidance protein) is a repulsive axon guidance cue. It is widely expressed in the developing hippocampus, glioma, and all histological types of the lung cancer cell. Draxin was shown to bind a 22-amino-acid peptide of the EGF domain of Netrin-1, and through Draxin/Netrin-1 interaction, Draxin may act as a secreted Netrin-1 antagonist. C1orf187 can inhibit or repel neurite outgrowth from dorsal spinal cord. It inhibits the stabilization of cytosolic beta-catenin (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist of Wnt signaling pathway (PMID: 29622850).