Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293T cells, HeLa cells, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86251-1-RR targets SASS6 in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag15917 Product name: Recombinant human SASS6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 141-490 aa of BC101026 Sequence: VECHEKEKHVNQLQTKVAVLEQEIKDKDQLVLRTKEAFDTIQEQKVVLEENGEKNQVQLGKLEATIKSLSAELLKANEIIKKLQGDLKTLMGKLKLKNTVTIQQEKLLAEKEEKLQKEQKELQDVGQSLRIKEQEVCKLQEQLEATVKKLEESKQLLKNNEKLITWLNKELNENQLVRKQDVLGPSTTPPAHSSSNTIRSGISPNLNVVDGRLTYPTCGIGYPVSSAFAFQNTFPHSISAKNTSHPGSGTKVQFNLQFTKPNASLGDVQSGATISMPCSTDKENGENVGLESKYLKKREDSIPLRGLSQNLFSNSDHQRDGTLGALHTSSKPTALPSASSAYFPGQLPNS Predict reactive species |
| Full Name | spindle assembly 6 homolog (C. elegans) |
| Calculated Molecular Weight | 657 aa, 74 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC101026 |
| Gene Symbol | SASS6 |
| Gene ID (NCBI) | 163786 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q6UVJ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Spindle assembly abnormal protein 6 homolog (SASS6) is a centrosomal protein that, together with PLK4, CEP135, CENPJ (also known as CPAP) and STIL, is involved in regulating the number of centrosomes in human cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SASS6 antibody 86251-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
