Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, Raji cells, A431 cells, HEK-293T cells, NIH/3T3 cells, PC-12 cells |
| Positive IHC detected in | Human lung cancer （Adenocarcinoma） Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86254-3-RR targets RPS3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag5273 Product name: Recombinant human RPS3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-264 aa of BC066926 Sequence: MAVSKNKRLTKGGKKGAKKKVVDPFSKKDWYDVKAPAMFNIRNIGKTLVTRTQGTKIASDGLKGRVFEVSLADLQNDEVAFRKFKLITEDVQGKNCLTNFHGMDLTRDKMCSMVKKWQTMIEAHVDVKTTDGYLLRLFCVGFTKKRNNQIRKTSYAQHQQVRQIRKKMMEIMTREVQTNDLKEVVNKLIPDSIGKDIEKACQSIYPLHDVFVRKVKMLKKPKFELGKLMELHGEGSSSGKATGDETGAKVERADGYEPPVQESV Predict reactive species |
| Full Name | ribosomal protein S3A |
| Calculated Molecular Weight | 30 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC066926 |
| Gene Symbol | RPS3A |
| Gene ID (NCBI) | 6189 |
| RRID | AB_3744755 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P61247 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RPS3a, a component of the ribosomal small subunit (40S), is a member of the A20 family of the deubiquitinating cysteine proteases. RPS3a is an inducing factor for oncogenic cellular transformation of Rat-1 cells by v-fos, and is highly expressed in most tumors including hepatocellular carcinoma and other cancers. On the other hand, RPS3a has also been described as a regulator of apoptosis and transcription factors.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPS3A antibody 86254-3-RR | Download protocol |
| IHC protocol for RPS3A antibody 86254-3-RR | Download protocol |
| WB protocol for RPS3A antibody 86254-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this product is a 5-star rating. The user reported successful use in immunofluorescence on HeLa human cells, employing a 1/200 dilution in PBS. Overall, the feedback is positive, indicating the antibody performed well for IF applications under these conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Céline (Verified Customer) (07-23-2026) | Work in immunofluorescence at the 1/200 dilution in PBS on Hela cells
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