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Tested Applications
| Positive WB detected in | mouse skin tissue |
| Positive IHC detected in | human skin cancer tissue, mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skin tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86310-1-RR targets Cytokeratin 79 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | keratin 79 |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | BC039148 |
| Gene Symbol | KRT79 |
| Gene ID (NCBI) | 338785 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q5XKE5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KRT79(Keratin 79, also known as K6L or KRT6L) is a type II epithelial keratin, which is mainly expressed in skeletal muscle, skin, scalp and other tissues and participates in maintaining the structural integrity of epithelial cells. KRT79 plays an important role in hair follicle development, and it is one of the marker genes of hair follicle outer root sheath and hair stromal cells (HG cells). Together with KRT17 and SOX9, it marks HG cells and participates in hair follicle regeneration and morphogenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Cytokeratin 79 antibody 86310-1-RR | Download protocol |
| IHC protocol for Cytokeratin 79 antibody 86310-1-RR | Download protocol |
| WB protocol for Cytokeratin 79 antibody 86310-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
