Cytokeratin 79 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of mouse skin using 86310-1-RR (same clone as 86310-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 86310-1-RR (KRT79 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86310-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 86310-1-RR (same clone as 86310-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 86310-1-RR (Cytokeratin 79 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86310-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 86310-1-RR (same clone as 86310-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86310-1-RR (Cytokeratin 79 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86310-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 86310-1-RR (same clone as 86310-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86310-1-RR (Cytokeratin 79 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86310-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skin using 86310-1-RR (same clone as 86310-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 79 antibody (86310-1-RR, Clone: 250927D8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86310-1-PBS in a different storage buffer formulation.

Cytokeratin 79 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 86310-1-PBS

Clone No.250927D8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CK-79, Cytokeratin-79, K6L, K79, KB38

---

Product Information

86310-1-PBS targets Cytokeratin 79 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: keratin 79
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC039148
• Gene Symbol: KRT79
• Gene ID (NCBI): 338785
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5XKE5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

KRT79(Keratin 79, also known as K6L or KRT6L) is a type II epithelial keratin, which is mainly expressed in skeletal muscle, skin, scalp and other tissues and participates in maintaining the structural integrity of epithelial cells. KRT79 plays an important role in hair follicle development, and it is one of the marker genes of hair follicle outer root sheath and hair stromal cells (HG cells). Together with KRT17 and SOX9, it marks HG cells and participates in hair follicle regeneration and morphogenesis.