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Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, NIH/3T3 cells |
| Positive IF-P detected in | mouse skin tissue, human breast cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86313-1-RR targets Cytokeratin 80 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | keratin 80 |
| Calculated Molecular Weight | 422 aa, 47 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC065180 |
| Gene Symbol | KRT80 |
| Gene ID (NCBI) | 144501 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q6KB66 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KRT80 is a human IF type II epithelial keratin gene involved in the formation of IF heterodimers in various epithelial cells. KRT80 is overexpressed in many neoplasms and plays an essential role in promoting cell proliferation, migration and invasiveness, and is associated with poor prognosis in cancer patients. KRT80 has three isoforms with predicted MW of 50 kDa, 47 kDa, and 54 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Cytokeratin 80 antibody 86313-1-RR | Download protocol |
| WB protocol for Cytokeratin 80 antibody 86313-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
