Tested Applications
| Positive WB detected in | A375 cells, A431 cells, Jurkat cells, U-937 cells, mouse testis tissue, rat testis tissue, rat lung tissue |
| Positive IF/ICC detected in | U-937 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86474-3-RR targets CHMP6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag9357 Product name: Recombinant human CHMP6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-201 aa of BC010108 Sequence: MGNLFGRKKQSRVTEQDKAILQLKQQRDKLRQYQKRIAQQLERERALARQLLRDGRKERAKLLLKKKRYQEQLLDRTENQISSLEAMVQSIEFTQIEMKVMEGLQFGNECLNKMHQVMSIEEVERILDETQEAVEYQRQIDELLAGSFTQEDEDAILEELSAITQEQIELPEVPSEPLPEKIPENVPVKARPRQAELVAAS Predict reactive species |
| Full Name | chromatin modifying protein 6 |
| Calculated Molecular Weight | 201 aa, 23 kDa |
| Observed Molecular Weight | 28-30 kDa |
| GenBank Accession Number | BC010108 |
| Gene Symbol | CHMP6 |
| Gene ID (NCBI) | 79643 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96FZ7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CHMP6 (charged multivesicular body protein 6) is a protein that plays a significant role in the formation of multivesicular bodies (MVBs) within cells. It is a component of the endosomal sorting complex required for transport III (ESCRT-III), which is involved in the sorting and transport of proteins within the cell. CHMP6 has been identified as a novel prognostic biomarker in bladder cancer, where it enhances cell survival and migration by regulating the cell cycle. (PMID: 40630215)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CHMP6 antibody 86474-3-RR | Download protocol |
| WB protocol for CHMP6 antibody 86474-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
