Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, HEK-293 cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86488-1-RR targets SECISBP2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag3541 Product name: Recombinant human SECISBP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 506-854 aa of BC036109 Sequence: NPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERKQRLQENAVGPAFTSDDTQDGESGGDDQFPEQAELSGPEGMDELISTPSVEDKSEEPPGTELQRDTEASHLAPNHTTFPKIHSRRFRDYCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLKKLKCVIISPNCEKIQSKGGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQDQFHKMVELTVAARQAYKTMLENVQQELVGEPRPQAPPSLPTQGPSCPAEDGPPALKEKEEPHYIEIWKKHLEAYSGCTLELEESLEASTSQMMNLNL Predict reactive species |
| Full Name | SECIS binding protein 2 |
| Calculated Molecular Weight | 854 aa, 95 kDa |
| Observed Molecular Weight | 95 kDa |
| GenBank Accession Number | BC036109 |
| Gene Symbol | SECISBP2 |
| Gene ID (NCBI) | 79048 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96T21 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SECISBP2 (also called SECIS-binding protein 2 or SBP2) is an RNA-binding protein that serves as the master regulator of selenoprotein synthesis in eukaryotes. It recognizes and binds the SECIS (SEC Insertion Sequence) stem-loop structure located in the 3′-UTR of all selenoprotein mRNAs, thereby converting the otherwise stop-coding UGA triplet into the 21st amino acid selenocysteine (Sec) during translation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SECISBP2 antibody 86488-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
