Tested Applications
| Positive WB detected in | mouse cerebellum tissue, mouse adipose tissue, rat cerebellum tissue |
| Positive IP detected in | mouse cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86519-4-RR targets ABCD2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag12872 Product name: Recombinant human ABCD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 371-740 aa of BC104901 Sequence: ADGEDGQKQVMVSERTEAFTTARNLLASGADAIERIMSSYKEVTELAGYTARVYNMFWVFDEVKRGIYKRTAVIQESESHSKNGAKVELPLSDTLAIKGKVIDVDHGIICENVPIITPAGEVVASRLNFKVEEGMHLLITGPNGCGKSSLFRILSGLWPVYEGVLYKPPPQHMFYIPQRPYMSLGSLRDQVIYPDSVDDMHDKGYTDQDLERILHNVHLYHIVQREGGWDAVMDWKDVLSGGEKQRMGMARMFYHKPKYALLDECTSAVSIDVEGKIFQAAKGAGISLLSITHRPSLWKYHTHLLQFDGEGGWRFEQLDTAIRLTLSEEKQKLESQLAGIPKMQQRLNELCKILGEDSVLKTIKNEDETS Predict reactive species |
| Full Name | ATP-binding cassette, sub-family D (ALD), member 2 |
| Calculated Molecular Weight | 740 aa, 83 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC104901 |
| Gene Symbol | ABCD2 |
| Gene ID (NCBI) | 225 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UBJ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ABCD2 is a member of the superfamily of ATP-binding cassette (ABC) transporters, and is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. ABCD2 plays a role in regulation of VLCFAs and energy metabolism, in the degradation and biosynthesis of fatty acids by beta-oxidation (PMID:29397936, 21145416).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ABCD2 antibody 86519-4-RR | Download protocol |
| WB protocol for ABCD2 antibody 86519-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
