ABCD2 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86519-4-RR (same clone as 86519-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86519-4-RR (ABCD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86519-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 86519-4-RR (same clone as 86519-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86519-4-RR (ABCD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86519-4-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse cerebellum using 86519-4-RR (same clone as 86519-4-PBS)

  IP result of anti-ABCD2 (IP:86519-4-RR, 4ug; Detection:86519-4-RR 1:2000) with mouse cerebellum tissue lysate 2000 ug. This data was developed using the same antibody clone with 86519-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86519-4-RR

  Biolayer interferometry (BLl) kinetic assays of 86519-4-RR against Human ABCD2 were performed. The affinity constant is below 1 pM.

ABCD2 Uni-rAb^® Recombinant Antibody for WB, IP, Indirect ELISA

Cat No. 86519-4-PBS

Clone No.251255A3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

ABCD 2, Adrenoleukodystrophy-like 1, Adrenoleukodystrophy-related protein, ALDRP, ATP-binding cassette sub-family D member 2

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Product Information

86519-4-PBS targets ABCD2 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag12872

  Product name: Recombinant human ABCD2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 371-740 aa of BC104901

  Sequence: ADGEDGQKQVMVSERTEAFTTARNLLASGADAIERIMSSYKEVTELAGYTARVYNMFWVFDEVKRGIYKRTAVIQESESHSKNGAKVELPLSDTLAIKGKVIDVDHGIICENVPIITPAGEVVASRLNFKVEEGMHLLITGPNGCGKSSLFRILSGLWPVYEGVLYKPPPQHMFYIPQRPYMSLGSLRDQVIYPDSVDDMHDKGYTDQDLERILHNVHLYHIVQREGGWDAVMDWKDVLSGGEKQRMGMARMFYHKPKYALLDECTSAVSIDVEGKIFQAAKGAGISLLSITHRPSLWKYHTHLLQFDGEGGWRFEQLDTAIRLTLSEEKQKLESQLAGIPKMQQRLNELCKILGEDSVLKTIKNEDETS

• Full Name: ATP-binding cassette, sub-family D (ALD), member 2
• Calculated Molecular Weight: 740 aa, 83 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC104901
• Gene Symbol: ABCD2
• Gene ID (NCBI): 225
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UBJ2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ABCD2 is a member of the superfamily of ATP-binding cassette (ABC) transporters, and is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. ABCD2 plays a role in regulation of VLCFAs and energy metabolism, in the degradation and biosynthesis of fatty acids by beta-oxidation (PMID:29397936, 21145416).