Importin Beta Recombinant monoclonal antibody

Importin Beta Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86630-3-RR

Clone No.251633D12

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

KPNB1, IMB1, Impnb, Importin 90, Importin subunit beta 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBAffinity

WB analysis using 86630-3-RR
Various lysates were subjected to SDS PAGE followed by western blot with 86630-3-RR (KPNB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 86630-3-RR
WB result of Importin Beta antibody (86630-3-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Importin Beta transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 86630-3-RR
Biolayer interferometry (BLl) kinetic assays of 86630-3-RR against Human Importin Beta were performed. The affinity constant is 1.02 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

A2780 cells, HeLa cells, HT-1080 cells, NIH/3T3 cells, C6 cells, mouse brain tissue, rat brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86630-3-RR targets Importin Beta in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag0122 Product name: Recombinant human KPNB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 409-612 aa of BC003572 Sequence: AMPTLIELMKDPSVVVRDTAAWTVGRICELLPEAAINDVYLAPLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLL Predict reactive species
Full Name	karyopherin (importin) beta 1
Calculated Molecular Weight	97 kDa
Observed Molecular Weight	90-97 kDa
GenBank Accession Number	BC003572
Gene Symbol	KPNB1
Gene ID (NCBI)	3837
RRID	AB_3745020
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q14974
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)