Tested Applications
| Positive WB detected in | IL-4 treated ACHN cells |
| Positive IF/ICC detected in | IL-4 treated ACHN cells |
| Positive FC (Intra) detected in | IL-4 treated ACHN cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86641-1-RR targets Phospho-STAT6 (Tyr641) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | signal transducer and activator of transcription 6, interleukin-4 induced |
| Calculated Molecular Weight | 100 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC005823 |
| Gene Symbol | STAT6 |
| Gene ID (NCBI) | 6778 |
| RRID | AB_3745023 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P42226 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. p-STAT6(Tyr641) is a biomarker of Th2-high inflammation, targeted by IL-4Rα blockers (dupilumab) and JAK1/3 inhibitors. High p-STAT6 in TAMs correlates with M2 polarization and radioresistance, blocking IL-4 or JAK1/3 decreases Tyr641 phosphorylation and restores anti-tumor immunity. (PMID: 37474750)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Phospho-STAT6 (Tyr641) antibody 86641-1-RR | Download protocol |
| IF protocol for Phospho-STAT6 (Tyr641) antibody 86641-1-RR | Download protocol |
| WB protocol for Phospho-STAT6 (Tyr641) antibody 86641-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Phospho-STAT6 (Tyr641) Recombinant monoclonal antibody (250940C12), catalog number 86641-1-RR, has 4 total citations. These appear in Current Issues in Molecular Biology, Journal of Cosmetic Dermatology, Tissue and Cell, and Cancers (Basel). The associated research fields include pancreatic adenocarcinoma progression via NF-κB/STAT6 signaling, skin barrier homeostasis through JAK-STAT modulation, postoperative pain relief using bone marrow stem cells, and glycolysis regulation in triple-negative breast cancer.
| Species | Application | Title |
|---|---|---|
Curr Issues Mol Biol ADGRG6 Promotes Pancreatic Adenocarcinoma Progression Through the NF-κB/STAT6 Axis and Modulation of the Tumor Immune Microenvironment.
| ||
J Cosmet Dermatol Dual-Targeting Oligopeptide-215 Regulates Skin Barrier Homeostasis Through Concurrent Modulation of JAK-STAT and NF-κB Signaling. | ||
Cancers (Basel) EIF3H Modulates Glycolysis Through LDHA Stabilization in Triple-Negative Breast Cancer. | ||
Tissue Cell Hepatocyte growth factor-overexpressing bone marrow stem cells relieve incision-induced postoperative pain. |
