Phospho-STAT6 (Tyr641) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86641-1-RR (same clone as 86641-1-PBS)

  Non-treated ACHN cells and IL-4 (HZ-1004) treated ACHN cells were subjected to SDS PAGE followed by western blot with 86641-1-RR (Phospho-STAT6 (Tyr641) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 86641-1-PBS in a different storage buffer formulation.

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  FC experiment of ACHN using 86641-1-RR (same clone as 86641-1-PBS)

  1X10^6 ACHN cells untreated (dashed lines) or treated with IL-4 treated which intracellularly stained with 0.13 ug Phospho-STAT6 (Tyr641) Recombinant monoclonal antibody (86641-1-RR, Clone:250940C12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026). This data was developed using the same antibody clone with 86641-1-PBS in a different storage buffer formulation.

Phospho-STAT6 (Tyr641) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 86641-1-PBS

Clone No.250940C12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

p STAT6 (Tyr641), Phospho STAT6, Phospho STAT6 (Tyr641), Phospho STAT6(Tyr641), Phospho-STAT6

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Product Information

86641-1-PBS targets Phospho-STAT6 (Tyr641) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: signal transducer and activator of transcription 6, interleukin-4 induced
• Calculated Molecular Weight: 100 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC005823
• Gene Symbol: STAT6
• Gene ID (NCBI): 6778
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P42226
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. p-STAT6(Tyr641) is a biomarker of Th2-high inflammation, targeted by IL-4Rα blockers (dupilumab) and JAK1/3 inhibitors. High p-STAT6 in TAMs correlates with M2 polarization and radioresistance, blocking IL-4 or JAK1/3 decreases Tyr641 phosphorylation and restores anti-tumor immunity. (PMID: 37474750)