Tested Applications
| Positive WB detected in | HT-29 cells, mouse colon tissue, Calu-3 cells, MCF-7 cells, rat colon tissue |
| Positive IP detected in | HT-29 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86697-1-RR targets Claudin 3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | claudin 3 |
| Calculated Molecular Weight | 220 aa, 23 kDa |
| Observed Molecular Weight | 20 kDa |
| GenBank Accession Number | BC016056 |
| Gene Symbol | Claudin 3 |
| Gene ID (NCBI) | 1365 |
| RRID | AB_3745061 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O15551 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Claudin-3 (CLDN3) is one of 27 known members of the claudin family. Together with occludin and other junctional adhesion molecules, the claudins form the tight-junctions (TJs) that regulate intercellular permeability. Altered CLDN3 expression has been linked to tumor progression in multiple tumor types.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for Claudin 3 antibody 86697-1-RR | Download protocol |
| WB protocol for Claudin 3 antibody 86697-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Claudin 3 Recombinant monoclonal antibody (251540A11), catalog number 86697-1-RR, has 4 total citations. These appear in Food Research International, Journal of Ethnopharmacology, DNA and Cell Biology, and European Journal of Pharmacology. The cited research spans hepatointestinal inflammation, gut microbiota and colitis, blood-milk barrier dysfunction in mastitis, and drug-induced liver injury via the gut-liver axis.
| Species | Application | Title |
|---|---|---|
Food Res Int Fermented grape pomace alleviates Salmonella typhimurium-induced Hepatointestinal inflammation: FXR mediates protection by orchestrating bile acid homeostasis and suppressing the colonic NLRP3 Inflammasome. | ||
J Ethnopharmacol Diallyl sulfide pretreatment protects against DSS-induced colitis by reshaping the gut microbiota and enhancing microbiota-associated tryptophan metabolism. | ||
Eur J Pharmacol Akkermansia muciniphila attenuates anti-tuberculosis drug-induced liver injury through gut-liver axis by promoting Nr4a1 transcription. | ||
DNA Cell Biol CPT1A Ameliorates Staphylococcus aureus-Induced Impairment of the Blood-Milk Barrier and Mitochondrial Oxidative Stress in Mastitis by Promoting NLRP3 Succinylation. |
