Filter: AllWBSandwich ELISA sample testSandwich ELISAIF/ICCCytometric bead array sample testCytometric bead arrayAffinity

WB analysis using 86719-3-RR (same clone as 86719-3-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 86719-3-RR (APEX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86719-3-PBS in a different storage buffer formulation.
Sandwich ELISA sample test of MP02621-2
Plasma of six individual healthy human donors was measured. The APEX1 concentration of detected samples was determined to be 0.22 ng/mL with a range of 0.11-0.41 ng/mL.
Sandwich ELISA sample test of MP02621-2
The mean APEX1 concentration was determined to be 141.21 ng/mL in A549 cell extract based on a 1.20 mg/mL extract load and 137.64 ng/mL in HCT 116 cell extract based on a 1.30 mg/mL extract load.
Sandwich ELISA standard curve of MP02621-2
Sandwich ELISA standard curve of MP02621-2, Human APEX1 Recombinant Matched Antibody Pair - PBS only. 86719-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0251. 86719-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.
IF Staining of MCF-7 using 86719-3-RR (same clone as 86719-3-PBS)
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using APEX1 antibody (86719-3-RR, Clone: 251693B6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86719-3-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 86719-3-RR (same clone as 86719-3-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APEX1 antibody (86719-3-RR, Clone: 251693B6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86719-3-PBS in a different storage buffer formulation.
Cytometric bead array sample test of MP02621-1
The mean APEX1 concentration was determined to be 101.1 ng/mL in Hela cell extract based on a 1.2 mg/mL extract load.
Cytometric bead array standard curve of MP02621-1
Cytometric bead array standard curve of MP02621-1, APEX1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86719-3-PBS. Detection antibody: 86719-2-PBS. Standard: Ag0251. Range: 0.312-40 ng/mL.
Affinity and Kinetic Characterization of 86719-3-PBS
Biolayer interferometry (BLl) kinetic assays of 86719-3-PBS against Human APEX1 were performed. The affinity constant is 1.33 nM.

APEX1 Recombinant monoclonal antibody, PBS Only (Capture)

APEX1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 86719-3-PBS

Clone No.251693B6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

APE, APE1, APE-1, APEN, APEX

Formulation: PBS Only

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

86719-3-PBS targets APEX1 as part of a matched antibody pair:

MP02621-1: 86719-3-PBS capture and 86719-2-PBS detection (validated in Cytometric bead array)

MP02621-2: 86719-3-PBS capture and 86719-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag0251 Product name: Recombinant human APEX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-318 aa of BC002338 Sequence: MPKRGKKGAVAEDGDELRTEPEAKKSKTAAKKNDKEAAGEGPALYEDPPDQKTSPSGKPATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCSENKLPAELQELPGLSHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGEEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTPYAYTFWTYMMNARSKNVGWRLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL Predict reactive species
Full Name	APEX nuclease (multifunctional DNA repair enzyme) 1
Calculated Molecular Weight	36 kDa
Observed Molecular Weight	36 kDa
GenBank Accession Number	BC002338
Gene Symbol	APEX1
Gene ID (NCBI)	328
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P27695
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

APEX1, also named as APE, APE1, HAP1 and REF-1, belongs to the DNA repair enzymes AP/ExoA family. It is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are in DNA repair and redox regulation of transcriptional factors. APEX nuclease is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. APEX1 is involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). When acetylated at Lys-6 and Lys-7, APEX1 stimulates the YBX1-mediated MDR1 promoter activity, leading to drug resistance. It also acts as an endoribonuclease involved in the control of single-stranded RNA metabolism. It plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, APEX1 plays a role in the rRNA quality control process during cell cycle progression.