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Cytokeratin 15 Recombinant monoclonal antibody (86761-3-RR)

Cytokeratin 15 Recombinant monoclonal antibody

Cytokeratin 15 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 86761-3-RR

Clone No.251445H2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

KRT15, CK 15, CK15, CK-15, Cytokeratin,Keratin

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

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Filter: AllWBIHC

WB analysis of HaCaT using 86761-3-RR
HaCaT cells were subjected to SDS PAGE followed by western blot with 86761-3-RR (Cytokeratin 15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 86761-3-RR
Mouse skin tissue were subjected to SDS PAGE followed by western blot with 86761-3-RR (KRT15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse skin using 86761-3-RR
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 86761-3-RR (Cytokeratin 15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse skin using 86761-3-RR
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 86761-3-RR (Cytokeratin 15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skin using 86761-3-RR
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 86761-3-RR (Cytokeratin 15 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skin using 86761-3-RR
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 86761-3-RR (Cytokeratin 15 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HaCaT cells, mouse skin tissue
Positive IHC detected in	mouse skin tissue, human skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86761-3-RR targets Cytokeratin 15 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	keratin 15
Calculated Molecular Weight	49 kDa
Observed Molecular Weight	46-50 kDa
GenBank Accession Number	BC002641
Gene Symbol	KRT15
Gene ID (NCBI)	3866
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P19012
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytokeratin 15 (CK15, encoded by KRT15) is a type I intermediate filament protein belonging to the keratin family. It is predominantly expressed in the basal and suprabasal layers of stratified epithelia, with particularly strong expression in the bulge region of the hair follicle, a well-characterized niche for epithelial stem cells. CK15 serves as a reliable marker for quiescent stem cells in various tissues, including the skin, mammary gland, and prostate, and its expression is often maintained during early stages of epithelial differentiation. In pathological contexts, CK15 is utilized as an immunohistochemical marker to distinguish basal cell carcinomas from other cutaneous neoplasms and to identify subsets of aggressive breast and prostate cancers. Its role in maintaining stem cell properties and tissue homeostasis makes it a valuable tool in both developmental biology and diagnostic pathology.