Cytokeratin 15 Recombinant monoclonal antibody, PBS Only

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• Cytokeratin 15 Recombinant monoclonal antibody, PBS Only (86761-3-PBS)

Cytokeratin 15 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 86761-3-PBS

Clone No.251445H2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

KRT15, CK 15, CK15, CK-15, Cytokeratin,Keratin

• 

  WB analysis of HaCaT using 86761-3-RR (same clone as 86761-3-PBS)

  HaCaT cells were subjected to SDS PAGE followed by western blot with 86761-3-RR (Cytokeratin 15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86761-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 86761-3-RR (same clone as 86761-3-PBS)

  Mouse skin tissue were subjected to SDS PAGE followed by western blot with 86761-3-RR (KRT15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86761-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse skin using 86761-3-RR (same clone as 86761-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 86761-3-RR (Cytokeratin 15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86761-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse skin using 86761-3-RR (same clone as 86761-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 86761-3-RR (Cytokeratin 15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86761-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin using 86761-3-RR (same clone as 86761-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 86761-3-RR (Cytokeratin 15 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86761-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin using 86761-3-RR (same clone as 86761-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 86761-3-RR (Cytokeratin 15 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86761-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

86761-3-PBS targets Cytokeratin 15 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: keratin 15
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC002641
• Gene Symbol: KRT15
• Gene ID (NCBI): 3866
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19012
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cytokeratin 15 (CK15, encoded by KRT15) is a type I intermediate filament protein belonging to the keratin family. It is predominantly expressed in the basal and suprabasal layers of stratified epithelia, with particularly strong expression in the bulge region of the hair follicle, a well-characterized niche for epithelial stem cells. CK15 serves as a reliable marker for quiescent stem cells in various tissues, including the skin, mammary gland, and prostate, and its expression is often maintained during early stages of epithelial differentiation. In pathological contexts, CK15 is utilized as an immunohistochemical marker to distinguish basal cell carcinomas from other cutaneous neoplasms and to identify subsets of aggressive breast and prostate cancers. Its role in maintaining stem cell properties and tissue homeostasis makes it a valuable tool in both developmental biology and diagnostic pathology.