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Zinc Alpha 2 Glycoprotein Recombinant monoclonal antibody, PBS Only (Detector) (86781-3-PBS)

Filter: AllWBSandwich ELISA sample testSandwich ELISAIHCAffinity

WB analysis of human plasma using 86781-3-RR (same clone as 86781-3-PBS)
human plasma were subjected to SDS PAGE followed by western blot with 86781-3-RR (AZGP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86781-3-PBS in a different storage buffer formulation.
Sandwich ELISA sample test of MP02669-2
Urine of six individual healthy human donors was measured. The ZAG/AZGP1 concentration of detected samples was determined to be 112.47 ng/mL with a range of 43.71-226.60 ng/mL.
Sandwich ELISA sample test of MP02669-2
Plasma of six individual healthy human donors was measured. The ZAG/AZGP1 concentration of detected samples was determined to be 137.18 ug/mL with a range of 49.90-221.70 ug/mL.
Sandwich ELISA standard curve of MP02669-2
Sandwich ELISA standard curve of MP02669-2, Human ZAG/AZGP1 Recombinant Matched Antibody Pair - PBS only. 86781-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg6736. 86781-3-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.
IHC staining of human breast cancer using 86781-3-RR (same clone as 86781-3-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86781-3-RR (Zinc Alpha 2 Glycoprotein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86781-3-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 86781-3-RR (same clone as 86781-3-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86781-3-RR (Zinc Alpha 2 Glycoprotein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86781-3-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 86781-3-RR
Biolayer interferometry (BLl) kinetic assays of 86781-3-RR against Human Zinc Alpha 2 Glycoprotein were performed. The affinity constant is 63.7 pM.

Zinc Alpha 2 Glycoprotein Recombinant monoclonal antibody, PBS Only (Detector)

Name: Zinc Alpha 2 Glycoprotein Recombinant monoclonal antibody, PBS Only (Detector)
Brand: Proteintech
SKU: 86781-3-PBS

Zinc Alpha 2 Glycoprotein Uni-rAb^® Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA

Cat No. 86781-3-PBS

Clone No.251605B9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA

Synonyms

AZGP1, Zinc-alpha-2-glycoprotein, ZA2G, ZAG, Zn alpha 2 glycoprotein

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

86781-3-PBS targets Zinc Alpha 2 Glycoprotein as part of a matched antibody pair:

MP02669-2: 86781-2-PBS capture and 86781-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg6736 Product name: Recombinant Human Zinc Alpha 2 Glycoprotein protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 21-298 aa of BC033830 Sequence: QENQDGRYSLTYIYTGLSKHVEDVPAFQALGSLNDLQFFRYNSKDRKSQPMGLWRQVEGMEDWKQDSQLQKAREDIFMETLKDIVEYYNDSNGSHVLQGRFGCEIENNRSSGAFWKYYYDGKDYIEFNKEIPAWVPFDPAAQITKQKWEAEPVYVQRAKAYLEEECPATLRKYLKYSKNILDRQDPPSVVVTSHQAPGEKKKLKCLAYDFYPGKIDVHWTRAGEVQEPELRGDVLHNGNGTYQSWVVVAVPPQDTAPYSCHVQHSSLAQPLVVPWEAS Predict reactive species
Full Name	alpha-2-glycoprotein 1, zinc-binding
Calculated Molecular Weight	298 aa, 34 kDa
Observed Molecular Weight	41 kDa
GenBank Accession Number	BC033830
Gene Symbol	Zinc Alpha 2 Glycoprotein
Gene ID (NCBI)	563
RRID	AB_3745114
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P25311
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Zinc-alpha-2-glycoprotein (AZGP1) is a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).